Rodolphe Marie

Rodolphe Marie, Associate Professor, DTU Nanotech, Technical University of Denmark

Rodolphe Marie

Biography:

Rodolphe Marie is currently an associate professor at the Department of Micro and Nanotechnology at the Technical University of Denmark (DTU Nanotech). He received his Engineer degree from the National Institute for Applied Sciences (INSA), France, in 1999 and a PhD degree at the Technical University of Denmark in 2004. His research interests are in nanofluidics for single molecules and bioimaging.

Invited talk:

Injection Molded Lab-On-A-Chip For Single Cell Genomics

Microfluidics devices can sort, manipulate and capture single cells. Those functionalities can be combined into single lab-on-a-chip platforms. One possible application is the single cell analysis and in particularly sample preparation for DNA sequencing. In such devices, individual cells are captured and lysed so that DNA and/or RNA is collected and amplified. In other devices, the fluidic channels cross section is reduced (nanofluidics) so they can be used to manipulate single DNA molecules for imaging [1]. Genomic DNA imaging allows mapping the human genome at a coarse resolution (1kb) [2] and is thus complementary to sequencing (1bp resolution). Thus far there has been only few attempts in fabricating a lab on a chip device capable of both single cell handling and genomic DNA imaging [3]. In a recent European efforts [Cellomatic FP7 HEALTH] our consortium have demonstrated an injection molded platform integrating microfluidics and nanofluidics. In particular, microfluidics channels with high aspect ratio (> 6) for single cell trapping and lysis [4] are combined with nanofluidics channels with low aspect ratio (down to 0.005) for DNA stretching [5]. In addition, the platform has a 150 mm lid allowing for high NA fluorescence imaging of single DNA molecules.